Title:
α−ガラクトースエピトープ発現ウイルス及びワクチンの作製方法
Document Type and Number:
Japanese Patent JP5699377
Kind Code:
B2
Abstract:
A method for producing an ±-Gal-expressing virus having enhanced immune response to viruses, without requiring the use of any enzyme; an influenza virus vaccine having a high effect (antigenicity), which is produced using an ±-Gal-expressing virus produced by the method; and others. Specifically disclosed are: a method for producing an ±-galactose epitope (Gal±1-3Gal²1-4GlcNAc -R: ±-Gal hereinafter)-expressing virus, which comprises the steps of:
(1) introducing an ±1,3-galactosyltransferase gene in an expressible condition into a cell line that does not express ±-Gal to obtain a cell line capable of expressing ±-Gal;
(2) inoculating a virus into the cell line capable of expressing ±-Gal to obtain a cell line infected with a virus; and
(3) culturing the cell line infected with the virus to obtain a virus expressing ±-Gal from the culture medium.
Inventors:
Haruko Ogawa
Kunitoshi Imai
Takahiro Tagami
Kunitoshi Imai
Takahiro Tagami
Application Number:
JP2011534305A
Publication Date:
April 08, 2015
Filing Date:
September 30, 2010
Export Citation:
Assignee:
Obihiro University of Agriculture and Veterinary Medicine
Centro Internacional de Agricultura Tropical
Centro Internacional de Agricultura Tropical
International Classes:
C12N7/00; A61K39/145; A61K39/165; A61K39/17; A61K39/20; A61K39/21; A61K39/255; A61K39/285; A61P31/12; A61P37/04; C12N7/04; C12N15/09
Foreign References:
| US20090123494 | ||||
| WO2008118487A2 |
Other References:
Blood,2002年,Vol.99,p.2477-2482
J. Virol.,2007年,Vol.81,p.9131-9141
Nature,1996年,Vol.379,p.85-88
Gene,1991年,Vol.108,p.193-200
Glycobiology,1993年,Vol.3,p.575-580
J. Virol.,2007年,Vol.81,p.9131-9141
Nature,1996年,Vol.379,p.85-88
Gene,1991年,Vol.108,p.193-200
Glycobiology,1993年,Vol.3,p.575-580
Attorney, Agent or Firm:
Patent Service Corporation Patent Office Sykes