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Title:
METHOD FOR PRODUCING LOW MOLECULAR WEIGHT CHITOSAN
Document Type and Number:
Japanese Patent JP2011236264
Kind Code:
A
Abstract:

To provide a method for producing low molecular weight chitosan excellent in safety and transfer efficiency as a carrier for transferring a gene and having little irregularity on the gene transfer activity, in good repeatability.

The method for producing the low molecular weight chitosan is characterized by comprising (1) a deacetylation process using a strong alkali, while using chitin as a raw material, (2) a cleaning process using endotoxin-free water, and (3) a molecular weight-lowering process using hydrogen peroxide. The weight-average molecular weight (Mw) of the low molecular weight chitosan is preferably 45 to 60 kDa, and the ratio (Mw/Mn) of the weight-average molecular weight (Mw) to the number-average molecular weight (Mn) is preferably 2 to 4.


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Inventors:
YAMAMURA AKIHIRO
KAWAGUCHI MITSUAKI
MATAHIRA YOSHIHARU
SATO TOMONORI
Application Number:
JP2010106330A
Publication Date:
November 24, 2011
Filing Date:
May 06, 2010
Export Citation:
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Assignee:
YAIZU SUISANKAGAKU IND CO LTD
International Classes:
C08B37/08; C12N15/09
Domestic Patent References:
JP2005176830A2005-07-07
JPS5633401B21981-08-04
JPH03164191A1991-07-16
JP2004263065A2004-09-24
JP2001031702A2001-02-06
Attorney, Agent or Firm:
Shigeru Matsui



 
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