PURPOSE: To improve yield of enzyme, by including a mold of a bacterium capable of producing a thermostable enzyme in heat-resistant gel and immobilizing the mold to the gel and bringing the immobilized particle into contact with a liquid culture medium under a culture condition of the mold.
CONSTITUTION: A mold of a bacterium capable of producing a thermostable enzyme (preferably heatstable, anaerobic bacterium) is included in heat-resistant gel (calcium alginate, carrageenan, polyacrylamide, polyvinyl alcohol, etc.) and immobilized. The prepared immobilized particles are brought into contact with a liquid culture medium under a culture condition of the mold. The gel used for inclusion and immoblization has preferably ≤1μ pore size of gel matrix.
HAGA RYOICHI
TSUCHIYA MASAMI
NISHIMURA YUSAKU
YAMAGUCHI TETSUO
| JPS54132294A | 1979-10-15 | |||
| JPS60227677A | 1985-11-12 |