Title:
METHOD FOR PURIFYING TERMINAL-MODIFIED TARGET NUCLEIC ACID
Document Type and Number:
Japanese Patent JP2023000571
Kind Code:
A
Abstract:
To improve the sensitivity of genetic analysis or improve detection efficiency for genetic mutations, by selectively purifying primers having modified molecules at their ends.SOLUTION: Provided is a method for purifying a target nucleic acid having a modified molecule at its end, in a method for detecting a target nucleic acid or determining a target nucleic acid based on the mobility of the nucleic acid in electrophoresis, the method comprising: subjecting a double-stranded nucleic acid having a modified molecule at the end of one of the nucleic acid strands to a heat denaturation treatment to form a single-stranded nucleic acid, and treating the resulting single-stranded nucleic acid with an exonuclease at a heat denaturation temperature to remove nucleic acids other than the nucleic acid strand having the modified molecule at the end.SELECTED DRAWING: Figure 5
Inventors:
GOTO YUSUKE
YOKOI TAKAHIDE
YOKOI TAKAHIDE
Application Number:
JP2021101476A
Publication Date:
January 04, 2023
Filing Date:
June 18, 2021
Export Citation:
Assignee:
HITACHI LTD
International Classes:
C12N15/10; C12Q1/6869
Attorney, Agent or Firm:
Patent Attorney Corporation Hiraki International Patent Office