Title:
METHOD FOR OBTAINING SPATIAL INFORMATION AND SEQUENCING INFORMATION OF m-RNA FROM TISSUE
Document Type and Number:
Japanese Patent JP2023046400
Kind Code:
A
Abstract:
To provide a method for sequencing and spatial decoding of m-RNA molecules of a tissue where the generation and decoding of a DNA bar code are done concomitantly.SOLUTION: A method for obtaining a spatial location and sequence information of an m-RNA target sequence on a tissue sample may include the steps of: a. providing a solid surface having at least one fiducial marker; b. attaching a plurality of anchor molecules comprising a photo-cleavable linker and an adapter unit to the solid surface; and c. binding scaffolding molecules comprising a unit capable of binding to the adapter unit of an anchor molecule, a poly-inosine unit having 5 to 30 inosine bases and a poly-adenine unit having 10 to 50 adenine bases to the anchor molecules.SELECTED DRAWING: None
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Inventors:
HANSUELI MEYER
ROBERT PINARD
THOMAS ROTHMANN
CHRIS NEHME
ROBERT PINARD
THOMAS ROTHMANN
CHRIS NEHME
Application Number:
JP2022151013A
Publication Date:
April 04, 2023
Filing Date:
September 22, 2022
Export Citation:
Assignee:
MILTENYI BIOTEC BV & CO KG
International Classes:
C12Q1/6869; C12N15/09; C12Q1/68; C12Q1/6874
Attorney, Agent or Firm:
Einzel Felix-Reinhard
Taku Morita
Junichi Maekawa
Hideo Nagashima
Ueshima class
Taku Morita
Junichi Maekawa
Hideo Nagashima
Ueshima class