Title:
トランス-ジヒドロキシシクロヘキサジエンカルボン酸および/またはその誘導体の改善された製造および単離のための方法、ならびに該方法に適した遺伝的に改変された微生物
Document Type and Number:
Japanese Patent JP2004525606
Kind Code:
A
Abstract:
In the production of genetically-modified, non-pathogenic organisms particularly an improved production and isolation of trans-dihydroxycyclohexadiene carboxylic acids and/or derivatives thereof, the synthesis of the transhydroxy cyclohexadiene carboxylic acids is performed in an enantiomer-pure form without detectable impurities with chorismate by cultivation of at least one genetically altered non-pathogenic organism which has an aromate embolism and has, in comparison with a corresponding, genetically not altered organism, an increased activity of the isochorismate synthase (EntC) and isochorismatase (EntB) and an activity of the 2,3 dihydroxybenzoate synthase (EntA) which is close to zero, or an increased activity of the isochorismatase (EntB) and a reduced activity of the isochorismate synthase (EntC) and an activity of the 2,3-dihydroxybenzoat-synthase (entA) of close to zero.
Inventors:
Dirk Franke
Georg Sprenger
Volker Lorbach
Ralph Tachols
Michael Muller
Marx Halfer
Rolf Muller
Jörg Temes
Georg Sprenger
Volker Lorbach
Ralph Tachols
Michael Muller
Marx Halfer
Rolf Muller
Jörg Temes
Application Number:
JP2002522517A
Publication Date:
August 26, 2004
Filing Date:
August 30, 2001
Export Citation:
Assignee:
Forschungszentrum Juelich GmbH
International Classes:
C12N15/09; C07C51/47; C07C51/48; C07C62/32; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/14; C12N9/90; C12P7/42; (IPC1-7): C12N15/09; C07C51/47; C07C51/48; C07C62/32; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P7/42
Attorney, Agent or Firm:
Toshio Yano
Toshiomi Yamazaki
Takuya Kuno
Einzel Felix-Reinhard
Reinhard Einsel
Toshiomi Yamazaki
Takuya Kuno
Einzel Felix-Reinhard
Reinhard Einsel