Title:
活性なβ-NGFを得るための方法
Document Type and Number:
Japanese Patent JP4634611
Kind Code:
B2
Abstract:
Preparation of biologically active beta-NGF comprises denaturing its inactive insoluble proform followed by renaturation and pro-sequence cleavage. Preparation of biologically active beta -NGF from its inactive insoluble proform, obtained by recombinant preparation in prokaryotic cells comprises: (a) dissolving ProNGF in its inactive insoluble form in a denaturating solution ; (b) transferring into a non- or weak denaturing solution where the denatured proNGF assumes a biologically active conformation, determined by the presence of disulfide bridges occurring in natural beta -NGF; and (c) cleaving the pro-sequence to obtain the active beta -NGF followed by its isolation.
Inventors:
Rudolph the liner
Rattenhol, Anke
Schwarz, Elisabeth
Grossman, Adelbert
Rattenhol, Anke
Schwarz, Elisabeth
Grossman, Adelbert
Application Number:
JP2000576009A
Publication Date:
February 16, 2011
Filing Date:
October 11, 1999
Export Citation:
Assignee:
Scil Proteins GmbH
International Classes:
C12N15/09; C12P21/06; A61K38/00; C07K1/107; C07K1/12; C07K14/48; C12N15/12
Domestic Patent References:
| JP6327489A | ||||
| JP9023883A | ||||
| JP7265092A |
Other References:
J. Biol. Chem., (1997), Vol. 272, No. 34, p. 21582-21588
J. Cellular Biochemistry, (1989), Vol. 40, p. 309-320
J. Cellular Biochemistry, (1989), Vol. 40, p. 309-320
Attorney, Agent or Firm:
Takumi Hayasaka