Title:
ウイルス不活性化ヒトγグロブリンGを生成するための方法
Document Type and Number:
Japanese Patent JP4070468
Kind Code:
B2
Abstract:
The gammaglobulin is extracted from a fraction isolated by fractionation with ethanol in the presence of a carbohydrate, and after reducing the content of contaminants with PEG, it is applied to an anionic resin exchange column, an effluent being obtained in which the PEG content is subsequently reduced by ultrafiltration and which is concentrated in order to carry out sequentially an optional treatment at an acid pH and at least one of the following steps of viral inactivation, consisting of pasteurisation and a treatment with solvent/detergent, the product afterwards being precipitated and washed with PEG in order to eliminate any chemical viral inactivation reagents and then, by solubilisation and change of pH, the protein contaminants, and finally purified by ultrafiltration to reduce the volume and the PEG content, then carrying out an optional virus filtration and subsequent concentration to a protein value of 5% or 10%.
Inventors:
Perelist Devert
Francisco Labaneda Jimenez
Matereza Lopez Hernandez
Francisco Labaneda Jimenez
Matereza Lopez Hernandez
Application Number:
JP2002009029A
Publication Date:
April 02, 2008
Filing Date:
January 17, 2002
Export Citation:
Assignee:
Grifols, Ese.
International Classes:
C07K1/14; A61K39/395; G01N33/48; A61L2/00; A61P37/02; B01D61/14; B01J41/20; C07K1/18; C07K1/30; C07K1/34; C07K16/00; C07K16/06
Domestic Patent References:
| JP11199511A |
Foreign References:
| WO1999033484A1 |
Attorney, Agent or Firm:
Masao Okabe
Nobuaki Kato
Kazuo
Shinichi Usui
Ikuo Fujino
Takao Ochi
Teruhisa Motomiya
Norimichi Takanashi
Nobuaki Kato
Kazuo
Shinichi Usui
Ikuo Fujino
Takao Ochi
Teruhisa Motomiya
Norimichi Takanashi