To obtain a moisture-curing type one-can type polyurethane resin composition which bears isocyanate group on its chain terminals and is excellent in initial adhesion strength, handleability, operability and storage stability obtained by allowing a specific polyol component to react with an organic polyisocyanate component.

(A) A polyol component containing an amine polyol prepared by adding an alkylene oxide such as ethylene oxide to an aliphatic amine of 2-24 carbon atoms such as tallow amine is allowed to react with (B) an organic polyisocyanate component such as diphenylmethane diisocyanate to give the objective moisture-curing type urethane prepolymer bearing isocyanate groups on its chain terminals, where the content of the above amine polyol is set in the range from 2 to 100wt.% based on the total polyol components.