多重ＲＮＡ誘導型ゲノム編集 - President and Fellows of Harvard College

Title:

多重ＲＮＡ誘導型ゲノム編集

Document Type and Number:

Japanese Patent JP7201294

Kind Code:

B2

Abstract:

Methods of multiplex genome engineering in cells using Cas9 is provided which includes a cycle of steps of introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to the target DNA and which guide the enzyme to the target DNA, wherein the one or more RNAs and the enzyme are members of a co-localization complex for the target DNA, and introducing into the cell a second foreign nucleic acid encoding one or more donor nucleic acid sequences, and wherein the cycle is repeated a desired number of times to multiplex DNA engineering in cells

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Inventors:

Church, George, Em.
DiCaro, James

Application Number:

JP2019238862A

Publication Date:

January 10, 2023

Filing Date:

December 27, 2019

Export Citation:

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Assignee:

President and Fellows of Harvard College

International Classes:

C12N15/09

Domestic Patent References:

JP2010131014A
JP2013520989A

Foreign References:

US20130052646

Other References:

Nucleic Acids Res.,2013年03月04日,Vol.41, No.7,pp.4336-4343
Science,2013年02月15日,Vol.339,pp.823-826, Supplementary Materials
Science,Vol.339,2013年02月15日,pp.819-823, Supplementary Materials
Nature Biotechnol.,2013年01月29日,Vol.31,pp.233-241
Nature,2009年,Vol.460,pp.894-898,METHODS
Cell, 2013年5月9日, vol.153, pp.910-918

Attorney, Agent or Firm:

Atsushi Nakajima
Kato Kazuho

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