Title:
変異型マルチ銅オキシダーゼ
Document Type and Number:
Japanese Patent JP6982867
Kind Code:
B2
Abstract:
To provide mutant McoP that has excellent thermal stability and storage stability, and can provide high oxidation reduction potential, and to provide nucleic acids encoding the same, as well as to provide electrode materials, cathode electrodes, and fuel cells comprising mutant McoP.SOLUTION: Provided is a mutant multicopper oxidase having multicopper oxidase activity, containing an amino acid sequence in which a methionine residue at a position corresponding to position 470 of SEQ ID NO: 2 is substituted with a hydrophobic amino acid residue, in (A) an amino acid sequence in which a methionine residue at position 470 in SEQ ID NO:2 is substituted with a hydrophobic amino acid residue, or in (B) an amino acid sequence having at least 80% sequence identity with the amino acid sequence shown in SEQ ID NO:2.SELECTED DRAWING: Figure 1
Inventors:
Eiichiro Takamura
Hiroaki Sakamoto
Takenori Satomura
Shinichiro Sue
Haruhiko Sakuraba
Hiroaki Sakamoto
Takenori Satomura
Shinichiro Sue
Haruhiko Sakuraba
Application Number:
JP2017252737A
Publication Date:
December 17, 2021
Filing Date:
December 28, 2017
Export Citation:
Assignee:
National University Corporation Fukui University
International Classes:
C12N15/09; C12N9/02; C12N15/53; H01M4/90; H01M8/16
Domestic Patent References:
| JP2013081410A | ||||
| JP2013081409A |
Other References:
Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J MolBiol. 1982 May 5;157(1):105-32.,1982年
Database Uniprot [online],Accession No. Q8ZWA8, 30-AUG-2017 uploaded, [retrieved on 2021-08-18], 配列情報, 〈URL: https://www.uniprot.org/uniprot/Q8ZWA8〉 Definition: Multicopper oxidase,2017年08月30日
櫻井 武、 科学研究費補助金研究成果報告書 研究課題番号:19350081、銅の解毒に関わるマルチ銅オキシダーゼの金属イオン特異性、酸素4電位還元と機能創製、平成22年4月5日、日本,2010年04月05日,
Database Uniprot [online],Accession No. Q8ZWA8, 30-AUG-2017 uploaded, [retrieved on 2021-08-18], 配列情報, 〈URL: https://www.uniprot.org/uniprot/Q8ZWA8〉 Definition: Multicopper oxidase,2017年08月30日
櫻井 武、 科学研究費補助金研究成果報告書 研究課題番号:19350081、銅の解毒に関わるマルチ銅オキシダーゼの金属イオン特異性、酸素4電位還元と機能創製、平成22年4月5日、日本,2010年04月05日,
Attorney, Agent or Firm:
Yoshihiro Akamatsu