Title:
【発明の名称】生体分子の標的用リガンドを同定するための核磁気共鳴の使用
Document Type and Number:
Japanese Patent JP3032301
Kind Code:
B2
Abstract:
A method for designing and forming a ligand which binds to a specific target molecule comprises the steps of: (a) identifying a first ligand moiety that binds to the target molecule using two-dimensional <15>N/<1>H NMR correlation spectroscopy; (b) identifying subsequent ligand moieties that bind to the target molecule using two-dimensional <15>N/<1>H NMR correlation spectroscopy; (c) forming a complex of the first and subsequent ligand moieties to the target molecule; (d) determining the three-dimensional structure of the complex and, thus, the spatial orientation of the first and subsequent ligand moieties on the target molecule; and (e) linking the first and subsequent ligand moieties to form a new ligand to maintain the spatial orientation of the ligand moieties.
Inventors:
Stephen D'Abri, Fiesque
Hajiyuk, Philip Jei
Hajiyuk, Philip Jei
Application Number:
JP51907497A
Publication Date:
April 17, 2000
Filing Date:
November 13, 1996
Export Citation:
Assignee:
Abbot Laboratories
International Classes:
C12Q1/00; G01N24/08; G01R33/465; G01N33/15; G01N33/50; G01N33/53; G01N33/542; G01N33/566; G01N33/58; G01N33/68; G01N; (IPC1-7): G01R33/465; G01N33/15; G01N33/566
Other References:
ＳＣＩＥＮＣＥ ｖｏｌ．２７４ ｐｐ．１５３１−１５３４
Attorney, Agent or Firm:
Yoshio Kawaguchi (1 person outside)