Title:
ハイブリッドモード刺激を伴う電圧モードを用いたナノポアベースの配列決定
Document Type and Number:
Japanese Patent JP6857237
Kind Code:
B2
Abstract:
A liquid voltage is applied to a first side of a lipid bilayer. The liquid voltage comprises a tag-reading period with a tag-reading voltage that tends to capture a tag into a nanopore in the lipid bilayer and an open-channel period with an open-channel voltage that tends to repel the tag. A pre-charging voltage source is connected to an integrating capacitor and a working electrode on a second side of the lipid bilayer during a pre-charging time period, such that the integrating capacitor and the working electrode are charged to a pre-charging voltage. The pre-charging voltage source is disconnected from the integrating capacitor and the working electrode during an integrating time period, such that a voltage of the integrating capacitor and a voltage of the working electrode may vary as a current flows through the nanopore. The pre-charging time period overlaps with a beginning portion of the tag-reading period.
Inventors:
Chen, Roger Jay A
Thien, Huy
Mayney, Jay William, Junior
Thien, Huy
Mayney, Jay William, Junior
Application Number:
JP2019514075A
Publication Date:
April 14, 2021
Filing Date:
September 14, 2017
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
G01N27/00; C12M1/00; C12Q1/6869
Domestic Patent References:
| JP2019531536A | ||||
| JP2019526043A | ||||
| JP2018501484A | ||||
| JP2019527819A |
Foreign References:
| WO2016099673A1 | ||||
| WO2017220732A1 |
Attorney, Agent or Firm:
Shinjiro Ono
Osamu Yamamoto
Toru Miyamae
Motoharu Nakanishi
Osamu Yamamoto
Toru Miyamae
Motoharu Nakanishi