Title:
新規なプライマーおよびその使用
Document Type and Number:
Japanese Patent JP7322063
Kind Code:
B2
Abstract:
Disclosed here is a composition comprising a primer that is (a) a loopable primer comprising a target-specific section, an adaptor section, and a stem-forming section, wherein the stem-forming section is hybridizable to a portion of the target-specific section to form a stem structure, or (b) a split primer comprising a first target-specific section, a second target-specific section, and an adaptor section positioned between the first target-specific section and the second target-specific section, or (c) a split-loopable primer comprising a first target-specific section, a second target-specific section, a stem-forming section positioned between the first target-specific section and the second target-specific section, and an adaptor section, or comprising a first adaptor section, a second adaptor section, a stem-forming section positioned between the first adaptor section and the second adaptor section, and a target-specific section. Also disclosed is a method for amplifying a target locus of interest from a template DNA, comprising at least two pre-amplification cycles using the loopable primer, the split primer and/or the split-loopable primer, wherein each amplification cycle comprises annealing the primer to the template DNA or pre-amplification product thereof and elongating the annealed primer. Further disclosed is a kit for amplifying a target locus of interest, comprising the loopable primer, the split primer, and/or the split-loopable primer.
Inventors:
Zimmerman, Bernhard
Swenerton, Ryan
Ru, Faye
Dashner, Scott
Seti, Himanshu
Swenerton, Ryan
Ru, Faye
Dashner, Scott
Seti, Himanshu
Application Number:
JP2020558864A
Publication Date:
August 07, 2023
Filing Date:
January 11, 2019
Export Citation:
Assignee:
Natera, Incorporated
International Classes:
C12Q1/686; C12Q1/6851; C12Q1/6855; C12Q1/6858; C12Q1/6869
Domestic Patent References:
| JP2013540451A |
Foreign References:
| WO2007011903A2 |
Other References:
Ke Chen et al.,Multiplex PCR with the Blunt Hairpin Primers for Next Generation Sequencing,Biotechnology and Bioprocess Engineering,2017年,22,347-351
Attorney, Agent or Firm:
Atsushi Aoki
Shinji Mihashi
Youichi Watanabe
Nakajima Masaru
Ryotaro Takei
Shinji Mihashi
Youichi Watanabe
Nakajima Masaru
Ryotaro Takei
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