Title:
核酸封入ＡＡＶ中空粒子
Document Type and Number:
Japanese Patent JP7440045
Kind Code:
B2
Abstract:
The present invention provides a method for manufacturing nucleic acid-encapsulating empty particles of the adeno-associated virus (AAV), the method comprising the following steps: (1) a step in which linear nucleic acid fragments are prepared, the nucleic acid fragments comprising (i) the sequence of the A region of an AAV inverted terminal repeat sequence (ITR), (ii) the sequence of the A' region of the AAV ITR, and (iii) the sequence of the D region and/or the sequence of the D' region of the AAV ITR; (2) a step in which the nucleic acid fragments from (1) are introduced into cells that generate AAV empty particles; and (3) a step in which the cells from (2) are cultured.
Inventors:
Naomi Okada
Hironori Okada
Yoshiyuki Miyakawa
Junichi Mineno
Hideto Chono
Hironori Okada
Yoshiyuki Miyakawa
Junichi Mineno
Hideto Chono
Application Number:
JP2022171573A
Publication Date:
February 28, 2024
Filing Date:
October 26, 2022
Export Citation:
Assignee:
Nippon Medical School
Takara Bio Co., Ltd.
Takara Bio Co., Ltd.
International Classes:
C12N7/01; C12N15/35; C12N15/864
Domestic Patent References:
| JP11506318A |
Foreign References:
| US20020182595 |
Attorney, Agent or Firm:
Norito Yamao
Norifumi Tomita
Manami Sasakura
Norifumi Tomita
Manami Sasakura
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