Title:
OLIGOSACCHARIDE OXIDASE, PRODUCTION OF OLIGOSACCHARIDE OXIDASE, DETERMINATION OF OLIGOSACCHARIDE, PRODUCTION OF OLIGOSACCHARIDE ACID AND DETERMINATION OF AMYLASE ACTIVITY
Document Type and Number:
Japanese Patent JP3310008
Kind Code:
B2
Abstract:
PURPOSE: To provide a new oligosaccharide oxidase useful for the determination of oligosaccharide and the production of oligosaccharide acid.
CONSTITUTION: The objective oligosaccharide oxidase has a molecular weight of 5.8-6.44m104, contains 1 molecule of FAD (flavine adenine dinucleotide) per 1 molecule of the oligosaccharide oxidase and has an isoelectric point of 4.2-4.4, an optimum pH of about 10 and an optimum temperature of about 50°C. The enzyme can be separated from the cultivation product obtained by cultivating a microbial strain capable of producing an oligosaccharide oxidase active to form an acid and hydrogen peroxide from an oligosaccharide and an enzyme by the hydrolysis corresponding to the oligosaccharide.
Inventors:
Tsai Ying-Jie
Hayashi Juntomi
Young child
Tadahiko Inukai
Hayashi Juntomi
Young child
Tadahiko Inukai
Application Number:
JP2722592A
Publication Date:
July 29, 2002
Filing Date:
January 17, 1992
Export Citation:
Assignee:
Biseiken Co., Ltd.
International Classes:
C12N9/04; C12N1/20; C12P7/58; C12Q1/26; C12Q1/40; C12Q1/54; C12R1/645; (IPC1-7): C12N9/04; C12N1/20; C12P7/58; C12Q1/26; C12Q1/40; C12Q1/54
Domestic Patent References:
| JP242980A | ||||
| JP60114193A | ||||
| JP5533862B1 |
Attorney, Agent or Firm:
Kiyomichi Kojima