Title:
PHOTOCHEMICAL LABEL FOR NUCLEIC ACID USING DIGOXIGENIN REAGENT
Document Type and Number:
Japanese Patent JP3253181
Kind Code:
B2
Abstract:
PURPOSE: To obtain a photochemical labeling reagent useful for genetic phatogonomy by adding a digoxigenin derivative, a spacer molecule, and a furocoumarin derivative to the reagent.
CONSTITUTION: A labeling reagent expressed by a formula, Dig-S-Fu, is synthesized. In the formula, Dig, S, and Fu respectively represent a digoxigenin derivative used as a hapten, a spacer molecule, and a furocoumarin derivative used as a photochemically bindable structure The digoxigenin derivative Dig is a chemically modified stroid derivative and contains a C-3-hydroxyalkyl amine group which is modified with a chemically binclable substituenl The spacer S is composed of polyalky amine, polyethylene glycol, or their combination. The photochemically bindable structure is especially furocoumarin, angelicin (isopsoralen) or psoralen, and their derivatives which photochemically react to nucleic acid. The binding order of the digoxigenin, spacer, and furocumarin can be decided arbitrarily. Usually, the Dig and S are first bound with each other and the Dig-S is made to react to the Fu. It is also possible to first form Fu-S and the Fu-S is made to react to the Dig.
Inventors:
Antonius Leverding
Gamal Kay Mikhail
Wolfgang Schewlinger
Gamal Kay Mikhail
Wolfgang Schewlinger
Application Number:
JP18220493A
Publication Date:
February 04, 2002
Filing Date:
June 29, 1993
Export Citation:
Assignee:
Bayer Aktiengesellschaft
International Classes:
C07D493/04; C07H21/00; C07J17/00; C07J19/00; C07J41/00; C12Q1/68; C12Q1/6816; G01N33/532; G01N33/58; (IPC1-7): G01N33/58; C12Q1/68
Domestic Patent References:
| JP61819A | ||||
| JP1503647A |
Attorney, Agent or Firm:
Heikichi Odashima