Title:
ポリヌクレオチドの分析の方法、システムおよびキット
Document Type and Number:
Japanese Patent JP2005508197
Kind Code:
A
Abstract:
Methods, systems, compositions and kits for improved detection of polynucleotides. In one aspect, there is provided a method for separating polynucleotides (such as DNA or RNA) using a liquid chromatographic separation device (such as a reverse phase column or an ion exchange column), contacting eluted polynucleotides with intercalating dye, and detecting (such as by fluorescence detection) dye bound to the eluted polynucleotides. The invention preferably uses a post-column reactor, such as a mixing tee, downstream of the separation column. Sensitivity of mutation detection by denaturing high performance liquid chromatography (DHPLC) is enhanced.
Inventors:
Regiender, Benjamin, Giunia
Rudolph, Joseph G, The Third
Marino, Michael A
Rudolph, Joseph G, The Third
Marino, Michael A
Application Number:
JP2003542656A
Publication Date:
March 31, 2005
Filing Date:
November 04, 2002
Export Citation:
Assignee:
Transgenic Incorporated
International Classes:
G01N27/62; B01D15/08; B01J20/285; C12M1/00; C12N15/09; C12N15/10; C12Q1/68; G01N21/78; G01N30/06; G01N30/26; G01N30/30; G01N30/72; G01N30/74; G01N30/88; G01N30/02; G01N30/84; (IPC1-7): C12N15/09; C12M1/00; C12Q1/68; G01N21/78; G01N27/62; G01N30/06; G01N30/26; G01N30/30; G01N30/48; G01N30/72; G01N30/74; G01N30/88
Attorney, Agent or Firm:
Heikichi Odashima