PURPOSE: The titled complex useful remedying hemorrhage of a patient of hemophilia, having raised preservation stability of the factor VIII and purification degree, obtained by bringing phospholipid into contact with blood coagulation factor.
CONSTITUTION: For example, phospholipid (e.g., phosphatidylcholine, or phosphatidic acid) is dissoved in a solvent (e.g., CHCl3, ethanol, etc.), the solvent is distilled away, so that a thin film of phospholipid is formed in the inner face of a container. The formed film is shaked, stirred, and destroyed to prepare particles. The particles are preferably treated with ultrasonic wave, and adjusted to ≤0.3μ particle diameters. The particles are blended and brought into contact with blood coagulation factor VIII, to give a complex of blood coagulation factor VIIIand phospholipid. The complex has 2W25 times as much activity as that of the factor VIII, and 88W96wt% recovery ratio. It has also high preservation stability. Intravenous injection is made possible by adjusting the complex to proper particle diameter.
MATSUDA HIROSHI
YOKOYAMA KAZUMASA
NISHIDA MASAYUKI
SUYAMA TADAKAZU
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