PREPARATION METHOD OF CANCER STEM CELL POPULATION, PREPARATION METHOD OF XENOGRAFT, SCREENING METHOD, METHOD FOR REDUCING miR-34a EXPRESSION QUANTITY AND CANCER STEM CELL PROLIFERATION INHIBITOR

Title:

PREPARATION METHOD OF CANCER STEM CELL POPULATION, PREPARATION METHOD OF XENOGRAFT, SCREENING METHOD, METHOD FOR REDUCING miR-34a EXPRESSION QUANTITY AND CANCER STEM CELL PROLIFERATION INHIBITOR

Document Type and Number:

Japanese Patent JP2016028569

Kind Code:

A

Abstract:

PROBLEM TO BE SOLVED: To provide a method enabling a cancer stem cell population to be efficiently isolated and cultivated reflecting a disease state of an intractable cancer or the like, and also to provide a method for preparing a xenograft, a screening method, a method for reducing a miR-34a expression quantity, and a cancer stem cell proliferation inhibitor.SOLUTION: The present invention relates to a preparation method of a cancer stem cell population. The preparation method comprises: implanting a cell mass containing a cancer stem cell into a non-human animal different from the species from which the stem cell is derived; forming a xenograft in the non-human animal; taking out the xenograft from the non-human animal; and cultivating the xenograft in a serum-free three-dimensional culture medium containing a growth factor which promotes the fission of the cancer stem cell, thereby forming an organoid.SELECTED DRAWING: Figure 1

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Inventors:

SAITO YOSHIMASA
SATO TOSHIAKI
SAITO HIDETANE
MURAMATSU TOSHIHIDE
KANAI YAE
OJIMA HIDETOMO

Application Number:

JP2015142164A

Publication Date:

March 03, 2016

Filing Date:

July 16, 2015

Export Citation:

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Assignee:

KEIO GIJUKU

International Classes:

C12N5/095; A61K38/00; A61P35/00; C12Q1/02; C12Q1/68

Foreign References:

WO2013062083A1	2013-05-02
WO2012168930A2	2012-12-13
WO2013081188A1	2013-06-06
US20100179213A1	2010-07-15

Other References:

JOURNAL OF HEPATO-BILIARY-PANCREATIC SCIENCES, vol. 19, JPN6019011248, 2012, pages 606 - 613, ISSN: 0004146182
JOURNAL OF HEPATO-BILIARY-PANCREATIC SCIENCES, vol. 21, JPN6019011250, 2014, pages 754 - 760, ISSN: 0004006180
ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 52, JPN6019011251, 2013, pages 3901 - 3905, ISSN: 0004006181
HEPATOLOGY, vol. 50, no. 2, JPN6019011252, 2009, pages 630 - 637, ISSN: 0004006182

Attorney, Agent or Firm:

Masayuki Masabayashi
Hayashi Ichiyoshi

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