To offer a production method of a living body tissue supplementation body in which while cells are efficiently seeded in a living body tissue supplementation material, it is possible to manufacture the living body tissue supplementation body for a short period of time by omitting exchange of a culture container according to growth of the cells, etc.
In the production method of the living body tissue supplementation body, a specified medium 4, adhering cells, and the living body tissue supplementation material are fed into the inside of the container 2 and the inside of the container 2 is maintained in a specified culture condition while the container 2 is rotated at a rotation speed of 2 rpm or more and 10 rpm or less around an approximately horizontal axis C.
TAKAMIYA YUJI
Masatake Shiga
Masakazu Aoyama
Suzuki Mitsuyoshi
Tadao Takashiba
Kunio Ueda