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Title:
PRODUCTION OF PROTEIN
Document Type and Number:
Japanese Patent JPH0538289
Kind Code:
A
Abstract:

PURPOSE: To provide a new recombinant DNA useful for efficiently obtaining a protein corresponding to an integrated protein structural gene.

CONSTITUTION: A recombinant DNA is obtained by inserting an alkaline protease promoter derived from a genomic gene of alkaline protease of yellow- green KOJI (yeast) mold and a protein structural gene into a vector DNA. The alkaline protease promoter derived from the genomic gene of the alkaline protease of the yellow-green KOJI mold has a base sequence expressed by the formula. The recombinant DNA is obtained by inserting the alkaline protease promoter derived from the genomic gene of the alkaline protease of the yellow-green KOJI mold, the objective protein structural gene and, as necessary, a terminator derived from the genomic gene of the alkaline protease of the yellow-green KOJI mold into a vector DNA.


Inventors:
USHIJIMA SHIGEOMI
TATSUMI HIROKI
MURAKAMI SEIJI
NAKANO EIICHI
MOTAI HIROSHI
ISHIDA YUTAKA
MURAKAMI KOJI
MAZAKI KOJI
KAWABE HARUHIDE
ARIMURA HIROBUMI
Application Number:
JP17629691A
Publication Date:
February 19, 1993
Filing Date:
April 17, 1991
Export Citation:
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Assignee:
SHOKUHIN SANGYO KOUSO KINOU HE
International Classes:
C12N1/15; C12N15/09; C12N15/80; C12P21/02; C12R1/69; (IPC1-7): C12N1/15; C12N15/80; C12P21/02
Attorney, Agent or Firm:
Yusuke Hiraki (2 outside)