PURPOSE: To purify crude secretin useful for treating peptic ulcer, etc. in high purity and yield, by using the change in the concentration of a hydrophilic organic solvent in a mixed solvent used as a mobile phase in the reversed-phase liquid chromatography.
CONSTITUTION: In purifying crude secretin by a reversed-phase liquid chromatography using a reversed-phase silica gel as a fixed phase and a mixed solvent of a hydrophilic organic solvent, e.g. isopropyl alcohol, with water as a mobile phase, the change in the concentration of the hydrophilic organic solvent is set within 20W90% range to carry out the adsorption, separation and elution of the aimed secretin. If the concentration of the isopropyl alcohol is ≤20%, the secretin is strongly adsorbed in th fixed phase and washed with the mixed solvent of the above-mentioned concentration. If the concentration of the isopropyl alcohol is ≥25%, the secretin is desorbed from the fixed phase and can be eluted with the mixed solvent of the above-mentioned concentration.
KITAKAJI SHIYOUJI
ITOU OSAMU
YOSHIDA SEIJI
OGAWA KOUICHI
KONISHI YUUSUKE
INT J REPT PROTIN RES
RECL TRAV CHIM PAYS-BAS