タンパク質の飽和標識用の試薬及び方法

Japanese Patent JP4318180

B2

A matched set of fluorescent dyes is provided, wherein each dye of the set is capable of covalent attachment to a protein and wherein each of the dyes has a molecular structure and a charge that is matched one with the other, such that relative electrophoretic mobility of a protein labeled with one dye of the set is the same as the electrophoretic mobility of the protein labeled with a different dye of the set. The matched set comprises at least two different fluorescent dyes of formula: wherein n is 1, 2, or 3; Z1 and Z2 independently represent the carbon atoms necessary to complete a phenyl or naphthyl ring system; one of groups R1 and R2 is a target bonding group; remaining group R1 or R2 is selected from -(CH2)4-W or -(CH2)r-H; group R3 is hydrogen, except when either R1 or R2 is -(CH2)r-H, in which case R3 is W; and W is selected from sulphonic acid and sulphonate. The invention also provides a method for saturation labeling of a protein with a fluorescent dye so as to label all available target amino acid, suitably cysteine, residues in the protein, thereby giving a single population of labeled protein molecules.

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Karen Williams
Stone, timothy
Simmons, Adrian Christopher
Sweet, Allison Claire
Fowler, Susan Janet

JP2004518895A

August 19, 2009

July 08, 2002

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GE Healthcare UK Limited

G01N33/58; C07K1/13; C09B23/00; C09B23/02; C09B67/22; G01N21/78; G01N33/68

JP10197481A
JP9127115A

WO2001011373A1

Arakawa Satoshi
Hirokazu Ogura
Nobukazu Ito
Toshihisa Kurokawa

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