Title:
反復的蛍光画像化
Document Type and Number:
Japanese Patent JP7440088
Kind Code:
B2
Abstract:
The invention relates to a method for multiplex staining of a biological sample involving the use of a buffer combination of blocking buffer, imaging buffer and elution buffer that allows for multiple staining rounds of biological samples. The blocking buffer comprises a compound that is capable of binding to hydrophobic binding sites non-specifically and a sulfhydryl-reactive compound. The imaging buffer is at neutral pH and comprises a radical scavenger, and the elution buffer is at pH lower than 4 and comprises a buffering component, a reducing agent and at least one compound disrupting hydrogen bonds. The invention further relates to buffers used in the practice of the method of the invention, and to a kit containing these buffers.
Inventors:
Gut, Gabriel
Perkman, Lucas
Hermann, Marcus
Perkman, Lucas
Hermann, Marcus
Application Number:
JP2020559536A
Publication Date:
February 28, 2024
Filing Date:
April 24, 2019
Export Citation:
Assignee:
Univel Zite Zurich
International Classes:
G01N33/48; G01N21/64; G01N33/53
Domestic Patent References:
| JP2013046576A |
Foreign References:
| WO2017137402A1 |
Other References:
ROGERS et al.,Thiol-reactive compounds prevent nonspecific antibody binding in immunohistochemistry,Laboratory Investigation,Vol.86,2006年,PP.526-533
Attorney, Agent or Firm:
Toshiaki Morimoto
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