NEW MATERIAL:The compound of formula I (X is a group which corresponds in the form of X-NH2 to secretin or a fragment devoid of 10 amino acids from its N-terminal; Y is residue corresponding in the form of Y-SH to β-galactosidase; Z is group of formula IIWformula IV).
USE: An enzyme-labeling antigen for the enzymatic immunoassay of secretin. Secretin can be determined in high specificity and sensitivity with a simple and easy operation with easy calibration.
PROCESS: The compound of formula I can be prepared by (1) reacting the crosslinking agent of formula V with secretin or secretin fragment of X-NH2 to introduce m-maleimidobenzoyl group to the N-terminal of secretin or secretin fragment, and (2) reacting the resultant compound of formula VI with β-D- galactosidase of formula Y-SH thereby adding the galactosidase to the maleimide group via the SH group of the galactosidase.
KATAYAMA KOUICHI
TSUCHIYA YUTAKA
YOSHINO HIROSHI
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