To provide a test method for predicting a skin-irritating property of a composition containing a high concentration of a lower alcohol by the use of a skin model.
After a substance to be tested is administered to a three-dimensional culture skin (skin model), air is blown to dry the substance to be tested to turn the lower alcohol speedily transpired, so that its effects are reduced to an negligible degree to perform evaluation. To be concrete, the test method is constituted of a process for administering a composition containing a high concentration of the lower alcohol to the skin model (process 1); a process for blowing air and drying an administered specimen (process 2); a process for cultivating the skin model after the administration (process 3); a process for evaluating a cell survival rate after the cultivation (process 4). It is possible by the test method to accurately predict the skin irritating property of the composition containing a high concentration of an alcohol in a short time.
MIYAMOTO KOJI