Title:
生物活性ペプチドの発現および精製のための可溶性タグ
Document Type and Number:
Japanese Patent JP5453261
Kind Code:
B2
Abstract:
Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.
Inventors:
Kay Chen
Linda Jane Decalolis
Stefan Earl Fahnestock
Tanya Maria Gruber
Lisa Diane Rice
Pierre U Louvre
Linda Jane Decalolis
Stefan Earl Fahnestock
Tanya Maria Gruber
Lisa Diane Rice
Pierre U Louvre
Application Number:
JP2010518341A
Publication Date:
March 26, 2014
Filing Date:
July 23, 2008
Export Citation:
Assignee:
E.I.DU PONT DE NEMOURS AND COMPANY
International Classes:
C12N15/09; C07K14/00; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02
Domestic Patent References:
| JP2006508639A | ||||
| JP1503117A | ||||
| JP2006516411A |
Foreign References:
| WO2003006494A1 |
Other References:
J. AM. CHEM. SOC.,2003年,Vol.125,p.9619-9628
Attorney, Agent or Firm:
Junji Yuda
Noriyuki Takebayashi
Noriyuki Takebayashi