Title:
ケト化合物の立体選択的酵素還元法
Document Type and Number:
Japanese Patent JP5852885
Kind Code:
B2
Abstract:
Stereoselective (preferably enantioselective) enzymatic reduction of ketocompounds to their corresponding chiral hydroxycompounds, comprises reducing the ketocompounds with an oxidoreductase, where: a polypeptide is used for the reduction of the ketocompounds; and the polypeptide includes the short-chain oxidoreductase and characteristics of e.g. (a) a N-terminal Rossmann-Fold GlyxxxGlyxGly, (b) a AsnAsnAlaGly-motif at position 86, (c) a catalytic triad consisting of Ser 139, Tyr 152 and Lys 156, and (d) a negatively charged amino acid residue at position 37. Stereoselective (preferably enantioselective) enzymatic reduction of ketocompounds to their corresponding chiral hydroxycompounds, comprises reducing the ketocompounds with an oxidoreductase, where: a polypeptide is used for the reduction of the ketocompounds; the polypeptide includes the short-chain oxidoreductase and characteristics of (a) a N-terminal Rossmann-Fold GlyxxxGlyxGly, (b) a AsnAsnAlaGly-motif at position 86, (c) a catalytic triad consisting of Ser 139, Tyr 152 and Lys 156, (d) a negatively charged amino acid residue at position 37 (according to the numerical system of 3alpha , 20beta -hydroxysteroid dehydrogenase), (e) a C-terminal motif in the dimerization domain [Val;Ile]-AspGly-[Gly;Ala]-Tyr-[Thr;Val;Cys]-[Ala;Thr]-[Gln;Val;Arg;Leu], (f) a (R)-alcoholdehydrogenase-signature at position 204-208, His-[Pro;Ala]-[Ile;Ala;Gln; Val]-GlyArg, (g) Val or Leu at position 160 (4 points downwards to Lys 156), and (h) Asp at position 178; and x is not defined.
Inventors:
Antigua, Gupta
Bob Kowa, Maria
Anke, Chainer
Bob Kowa, Maria
Anke, Chainer
Application Number:
JP2011552434A
Publication Date:
February 03, 2016
Filing Date:
March 03, 2010
Export Citation:
Assignee:
CAMBREX EAP GAME BAHA
International Classes:
C12N9/02; C12N15/09; C12P7/04; C12P7/24; C12P41/00
Domestic Patent References:
| JP2009502148A | ||||
| JP2008529542A | ||||
| JP2004527251A |
Foreign References:
| WO2002012544A1 |
Other References:
FEBS Letters,1999年,vol.445,pp.261-264
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY,1997年 1月 1日,V414,pp.403-415
BIOCHEMISTRY,1995年 5月 9日,V34 N18,pp.6003-6013
Database UNIPROT, [online],2003年 6月 1日,Q81AU6,[平成26年3月10日検索],URL,http://www.uniprot.org/uniprot/Q81AU6.txt?version=1
Database UNIPROT,[online],2006年 8月22日,Q11G59,[平成26年3月10日検索],URL,http://www.uniprot.org/uniprot/Q11G59.txt?version=1
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY,1997年 1月 1日,V414,pp.403-415
BIOCHEMISTRY,1995年 5月 9日,V34 N18,pp.6003-6013
Database UNIPROT, [online],2003年 6月 1日,Q81AU6,[平成26年3月10日検索],URL,http://www.uniprot.org/uniprot/Q81AU6.txt?version=1
Database UNIPROT,[online],2006年 8月22日,Q11G59,[平成26年3月10日検索],URL,http://www.uniprot.org/uniprot/Q11G59.txt?version=1
Attorney, Agent or Firm:
Seiji Ohno
Keiko Kanemoto
Reiko Tanaka
Yuko Matsuniya
Ken Kitano
Natsuki Ito
Umeda Shinsuke
Koji Morita
Keiko Kanemoto
Reiko Tanaka
Yuko Matsuniya
Ken Kitano
Natsuki Ito
Umeda Shinsuke
Koji Morita