Title:
STORAGE OF SERINE-RACEMASE
Document Type and Number:
Japanese Patent JPS5816677
Kind Code:
A
Abstract:
PURPOSE: To store serine.racemase contained in microbial cells, for a long period, without lowering the enzymatic activity, by suspending the microbial cells in a specific aqueous solution and freezing the suspension.
CONSTITUTION: Cells of bacteria belonging to Pseudomonas putida or Aeromonas punctate subsp. caviae and containing serine.racemase, e.g. Pseudomonas putida IFO 12996, are suspended in a ≥0.0001%, preferably 0.001W0.1% aqueous solution of pyridoxal phosphate at a concentration of 5W200g/l preferably 30W 100g/l in terms of dried cell. After adjusting the pH of the suspension to preferably 7W9, the suspension is frozen at -5W-50°C, preferably -10W-30°C.
Inventors:
MAKIGUCHI NOBUYOSHI
FUKUHARA NOBUHIRO
MATSUMOTO TOSHIO
FUKUHARA NOBUHIRO
MATSUMOTO TOSHIO
Application Number:
JP11441581A
Publication Date:
January 31, 1983
Filing Date:
July 23, 1981
Export Citation:
Assignee:
MITSUI TOATSU CHEMICALS
International Classes:
C12N9/96; C12N1/20; C12R1/01; C12R1/40; (IPC1-7): C12N1/20; C12N9/96