Title:
SYPHILIS TREPONEMA-FUSED DNA SEQUENCE AND EXPRESSION OF SYPHILIS TREPONEMA ANTIGEN
Document Type and Number:
Japanese Patent JP3536635
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain the subject new sequence, wherein a DNA sequence encoding a signal peptide-added syphilis treponema surface antigen is fused with a DNA sequence encoding a secretion-related enzyme, affording an antigen useful as e.g. an immunoassay reagent enabling highly sensitive assay.
SOLUTION: This sequence is a new syphilis treponema-fused DNA sequence, wherein, a DNA sequence encoding a signal peptide-added syphilis treponema surface antigen is fused with a DNA sequence encoding a secretion-related enzyme, and useful for the production of a large amount of a lipid-bound and highly reactive syphilis treponema antigen. By using this antigen as an immunoassay reagent, the syphilis treponema can be assayed in a more specific and highly sensitive way. This DNA sequence is obtained by specifically amplifying the DNA sequence for the signal-syphilis treponema antigen in the native DNA sequence for syphilis treponema, through a PCR technique using a primer and fusing the resultant DNA sequence for the signal-syphilis treponema antigen with the DNA sequence for a secretory enzyme through e.g. ligation.
Inventors:
Fujimura, Katsuya
Hara, Yasuhiro
Ito, Satoru
Hara, Yasuhiro
Ito, Satoru
Application Number:
JP36763897A
Publication Date:
June 14, 2004
Filing Date:
December 29, 1997
Export Citation:
Assignee:
FUJIREBIO INC
International Classes:
G01N33/53; C07K14/20; C07K19/00; C12N1/21; C12N15/00; C12N15/09; C12P21/02; G01N33/577; C12R1/01; C12R1/19; (IPC1-7): C12N15/09; C12P21/02