Title:
三次元軟骨培養物
Document Type and Number:
Japanese Patent JP3599341
Kind Code:
B2
Abstract:
The present invention relates to a method of stimulating the proliferation and appropriate cell maturation of a variety of different cells and tissues in three-dimensional cultures in vitro using TGF- beta in the culture medium. In accordance with the invention, stromal cells, including, but not limited to, chondrocytes, chondrocyte-progenitors, fibroblasts, fibroblast-like cells, umbilical cord cells or bone marrow cells from umbilical cord blood are inoculated and grown on a three-dimensional framework in the presence of TGF- beta . Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal cells and connective tissue proteins naturally secreted by the stromal cells attach to and substantially envelope the framework composed of a biocompatible non-living material formed into a three-dimensional structure having interstitial spaces bridged by the stromal cells. The living stromal tissue so formed provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture and/or cultures implanted in vivo. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts in vivo.
Inventors:
Percio, Anthony F.
Jimbar, Michael
Dunkelman, Noshin
Norton, Gale Kay.
Norton, Brian A.
Jimbar, Michael
Dunkelman, Noshin
Norton, Gale Kay.
Norton, Brian A.
Application Number:
JP50130896A
Publication Date:
December 08, 2004
Filing Date:
June 06, 1995
Export Citation:
Assignee:
TJ Smith and Nephew Limited
International Classes:
C12Q1/02; A61B19/00; A61K35/28; A61L27/00; A61L27/38; A61L27/60; C12N5/00; C12N5/071; C12N5/077; C12N5/0789; G01N33/50; A61B10/00; A61B10/02; A61F2/00; A61F2/04; A61F2/06; A61F2/08; A61K35/12; (IPC1-7): C12N5/06; A61L27/00
Domestic Patent References:
| JP59192364A | ||||
| JP3502579A | ||||
| JP4501657A | ||||
| JP4505717A |
Other References:
Progress in Growth Factor Research,Vol.4,pp.45-68(1992)
The Journal of Bone and Joint Surgery,Vol.76-A,No.4,pp.579-592(April 1994)
The Journal of Bone and Joint Surgery,Vol.76-A,No.4,pp.579-592(April 1994)
Attorney, Agent or Firm:
Shintaro Nogawa