Title:
【発明の名称】融合蛋白質の製造方法
Document Type and Number:
Japanese Patent JP3051162
Kind Code:
B2
Abstract:
Disclosed are methods of producing fusion proteins including those with dual biological activities. These methods include the provision of first and second DNA sequence encoding a first and second polypeptide, respectively, the digestion of the first DNA sequence at a restriction site adjacent its 5' terminus, and the ligation of a linker/adapter sequence (1/a) to the restricted end of the first DNA sequence, thereby forming a cassette. The 1/a includes, at one end, that portion of the first DNA sequence extending from its terminus nearest the restriction site to the restriction site, and at the other end, one side of a splice site. A eucaryotic host cell is transfected with the cassette and the second DNA sequence having, at one end, one side of a splice site compatible with the side of the splice site on the 1/a. The transfected host cell is cultured to express the transfected DNA as a single chain fusion protein.
Inventors:
Gillies, Steven Di.
Application Number:
JP51247490A
Publication Date:
June 12, 2000
Filing Date:
August 01, 1990
Export Citation:
Assignee:
Abbott Laboratories
International Classes:
C07K16/30; C07K16/46; C12N15/11; C12N15/09; C12N15/62; C12P21/02; C12P21/08; A61K38/00; C12R1/91; (IPC1-7): C12P21/08; C12N15/09
Attorney, Agent or Firm:
Seiya Fujino