Title:
【発明の名称】遺伝的ワクチンおよび酵素の非確率論的生成
Document Type and Number:
Japanese Patent JP2003524392
Kind Code:
A
Abstract:
Disclosed is a rapid and facilitated method of producing from a parental template polynucleotide, a set of mutagenized progeny polynucleotides whereby at each original codon position there is produced at least one substitute codon encoding each of the 20 naturally encoded amino acids. Accordingly, there is also provided a method of producing from a parental template polypeptide, a set of mutagenized progeny polypeptides wherein each of the 20 naturally encoded amino acids is represented at each original amino acid position. The method provided is termed site-saturation mutagenesis, or simply saturation mutagenesis, and can be used in combination with other mutagenization processes, such as, for example, a process wherein two or more related polynucleotides are introduced into a suitable host cell such that a hybrid polynucleotide is generated by recombination and reductive reassortment. Also provided are vector and expression vehicles including such polynucleotides, polypeptides expressed by the hybrid polynucleotides and a method for screening for hybrid polypeptides.
Inventors:
Short, Jay, M.
Application Number:
JP2000597406A
Publication Date:
August 19, 2003
Filing Date:
February 04, 2000
Export Citation:
Assignee:
Diversa corporation
International Classes:
C12N15/09; A61K38/00; A61K39/00; A61K39/39; A61K48/00; A61P33/06; C07K14/445; C12N1/15; C12N1/19; C12N1/21; C12N5/02; C12N5/10; C12N9/00; C12N9/16; C12N9/24; C12N9/38; C12N15/01; C12N15/10; C12P21/02; C12Q1/02; C12Q1/68; G01N33/15; G01N33/50; G01N33/53; G01N33/566; (IPC1-7): C12N15/09; A61K39/00; A61K39/39; A61K48/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02; C12Q1/02; C12Q1/68; G01N33/15; G01N33/50; G01N33/53; G01N33/566
Attorney, Agent or Firm:
Yusuke Hiraki (1 person outside)