Title:
【発明の名称】細胞および組織の三次元培養系
Document Type and Number:
Japanese Patent JPH04501657
Kind Code:
A
Abstract:
The present invention relates to a three-dimensional cell culture system which be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts actively growing on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.
Inventors:
Norton, Gale, K
Norton, Bryan, A
Norton, Bryan, A
Application Number:
JP50940289A
Publication Date:
March 26, 1992
Filing Date:
September 07, 1989
Export Citation:
Assignee:
Mallow-Tech Incorporated
International Classes:
C12N15/02; A61K49/00; A61L27/00; A61L27/38; A61L27/60; C12M3/00; C12N5/00; C12N5/02; C12N5/071; C12N5/10; C12N15/09; C12Q1/02; C12Q1/18; G01N33/15; G01N33/50; A61K35/12; (IPC1-7): A61K49/00; C12M3/00; C12N5/06; C12N5/08; C12N5/10; C12N15/06; C12Q1/18
Attorney, Agent or Firm:
Yusuke Hiraki (2 outside)