Title:
AGENT AND METHOD FOR DETECTING HYDROGEN PEROXIDE, SUBSTRATE CONTAINING SAME, PEROXIDASE AND SUBSTANCE HAVING PEROXIDASE LIKE ACTION
Document Type and Number:
Japanese Patent JPS5813398
Kind Code:
A
Abstract:
Composition for the detection of (a) hydrogen peroxide or hydrogen peroxide forming substrates or (b) peroxidases or peroxidatively reacting substances which composition comprises a substrate capable of coupling and an oxidizable substance known to undergo the Trinder reaction, and (a) a peroxidase or (b) hydrogen peroxide wherein the substrate capable of coupling is a compound of the formula (I) in which n and m are individually selected from whole numbers of 1 to 4, X and Y, which can be the same or different represent a valence bond or a phenylene radical, R1 and R2 are individually selected from carboxyl or sulfonic acid groups and one of R1 and R2 can also be hydrogen or lower alkyl, and R3 and R4 are individually selected from hydrogen and alkyl radicals of up to 6 carbon atoms; and/or a salt of such compound. The present invention also provides a method wherein the composition is brought together with a sample and the coloration obtained is evaluated visually or with a photometer.
Inventors:
URURITSUHI TOREEGAA
BERUNBUARUTO ZOYUKA
HANSU RANGE
BERUNBUARUTO ZOYUKA
HANSU RANGE
Application Number:
JP10404182A
Publication Date:
January 25, 1983
Filing Date:
June 18, 1982
Export Citation:
Assignee:
BOEHRINGER MANNHEIM GMBH
International Classes:
C07C67/00; C07C227/00; C07C229/38; C07C301/00; C07C309/45; G01N33/50; C07C309/46; C12Q1/28; (IPC1-7): C07C101/16; C07C101/42; C07C143/56; C12Q1/28; G01N33/52
Domestic Patent References:
| JPS5637556A | 1981-04-11 | |||
| JPS56133660A | 1981-10-19 |
Attorney, Agent or Firm:
Toshio Yano