Title:
ゲンチオオリゴ糖の製造における耐熱性β－グルコシダーゼの使用
Document Type and Number:
Japanese Patent JP7297924
Kind Code:
B2
Abstract:
An application of heat-resistant β-glucosidase TSBGl in preparation of gentiooligosaccharide. The heat-resistant β-glucosidase TSBGl from Thermotoga sp.KOL6 takes Bacillus subtilis WSH11 as an expression host, so that high-efficiency expression of the tsbgl gene in bacillus subtilis is achieved. The optimum temperature of the β-glucosidase TSBGl is 90℃, the optimum pH is 6.0, and the β-glucosidase TSBGl has relatively high thermostability at 90℃. The β-glucosidase is added to a reaction system employing 1200 g/L of glucose as a substrate, an enzyme reaction is carried out under a pH of 6.0 at 90℃, and the yield of the gentiooligosaccharide reaches 178.2 g/L. The β-glucosidase meets requirements for industrial applications such as foods and can be applied to industrial production of the gentiooligosaccharide.
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Inventors:
Wu Jing
Xia Wei
Rei Mori
yellow swallow
Xia Wei
Rei Mori
yellow swallow
Application Number:
JP2021558012A
Publication Date:
June 26, 2023
Filing Date:
August 18, 2020
Export Citation:
Assignee:
JIANGNAN UNIVERSITY
International Classes:
C12N15/56; C12N1/21; C12N9/24; C12N15/63; C12P19/00
Domestic Patent References:
| JP9000259A |
Foreign References:
| CN109628435A | ||||
| CN109371006A | ||||
| CN107236696A | ||||
| CN108384769A | ||||
| WO2018187524A1 | ||||
| US4725535 |
Other References:
MHAINDARKAR D. et al.,Loss of a conserved salt bridge in bacterial glycosyl hydrolase BgIM-G1 improves substrate binding in temperate environments,Communications Biology, 2018, 1: 171, DOI: 10.1038/s42003-018-0167-7
Attorney, Agent or Firm:
Sonoda & Kobayashi Patent Attorneys Corporation
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