Title:
DETECTION METHOD OF TLR STIMULATING SUBSTANCE
Document Type and Number:
Japanese Patent JP2016208851
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide methods of sharply and rapidly detecting Listeria bacteria included in samples, such as environmental water, a flagellin derived from Salmonella bacteria, a lipopeptide derived from Mycoplasma bacteria, or a lipopolysaccharide derived from pathogenic bacteria.SOLUTION: By using a method comprising culturing under the presence of a sample a macrophage cell in which an expression cassette having a promoter sequence of IL-8 gene and a reporter gene sequence operably linked to the downstream of the promoter sequence is introduced, Listeria bacteria in the sample, a flagellin derived from Salmonella bacteria, a lipopeptide derived from Mycoplasma bacteria, or a lipopolysaccharide derived from pathogenic bacteria can be detected simply and highly sensitively.SELECTED DRAWING: None
Inventors:
KIMURA YUTAKA
AIBA SETSUYA
AIBA SETSUYA
Application Number:
JP2015092504A
Publication Date:
December 15, 2016
Filing Date:
April 30, 2015
Export Citation:
Assignee:
UNIV TOHOKU
International Classes:
C12Q1/02; G01N33/569
Domestic Patent References:
| JP2010538628A | 2010-12-16 |
Foreign References:
| WO2012002507A1 | 2012-01-05 |
Other References:
NOVUS BIOLOGICALS NBP2-26291, JPN6019007104, 1 June 2014 (2014-06-01)
MOLECULAR IMMUNOLOGY, vol. Vol.47, JPN6019007106, 2009, pages 614 - 622
TOXICOLOGICAL SCIENCES, vol. 124, no. 2, JPN6019007108, 2011, pages 359 - 369
MOLECULAR IMMUNOLOGY, vol. Vol.47, JPN6019007106, 2009, pages 614 - 622
TOXICOLOGICAL SCIENCES, vol. 124, no. 2, JPN6019007108, 2011, pages 359 - 369
Attorney, Agent or Firm:
Masaki Hirota
Seiji Ozawa
Yusaku Tokai
Kazuhiro Matsuda
Makoto Horiuchi
Masako Yamauchi
Shuichi Sonomoto
Akihiro Yamamura
Seiji Ozawa
Yusaku Tokai
Kazuhiro Matsuda
Makoto Horiuchi
Masako Yamauchi
Shuichi Sonomoto
Akihiro Yamamura