Title:
The manufacturing method of a patient custom-made type Glioblastoma animal model, and the use of this
Document Type and Number:
Japanese Patent JP6026653
Kind Code:
B2
Abstract:
The present invention relates to a method for producing a glioblastoma mouse model, comprising the steps of: (a) dividing a glioblastoma tissue, isolated from a patient, into 4 or more sections, and collecting one or more pieces from each of the sections; (b) dissociating a mixture of the collected pieces into glioblastoma cells as single cells; and (c) orthotopically transplanting a graft sample, which contains the glioblastoma cells obtained in step (b), into the brain of an immunodeficient mouse. The present invention also relates to a mouse model produced by the method, a method of screening a glioblastoma therapeutic agent using the mouse model, and a method of providing information for selection of a patient-specific glioblastoma therapy using the mouse model. The glioblastoma mouse model according to the present invention shows the same genetic, morphological and pathological characteristics as those of the parental tumor, and thus it makes possible to screen a patient-specific glioblastoma therapeutic agent or select a safer and more effective patient-specific glioblastoma therapy.
Inventors:
Ham Do Hyun
Chin Ju Young
Ju Kyung Min
Chin Ju Young
Ju Kyung Min
Application Number:
JP2015518340A
Publication Date:
November 16, 2016
Filing Date:
June 21, 2013
Export Citation:
Assignee:
SAMSUNG LIFE PUBLIC WELFARE FOUNDATION
International Classes:
A01K67/027; C12N5/09; C12Q1/04; G01N33/15; G01N33/50
Domestic Patent References:
| JP2008536498A | ||||
| JP2008074321A |
Other References:
BROADLEY, K.W. et al.,Side population is not necessary or sufficient for a cancer stem cell phenotype in glioblastoma multiforme.,Stem Cells,2011年,Vol.29 No.3,p.452-461,"Materials and Methods"
FEI, X.F. et al.,Development of clinically relevant orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma through surgical tumor tissues injection with trocar.,J. Exp. Clin. Cancer Res. ,2010年,Vol.29 No.1,p.1-8
FEI, X.F. et al.,Development of clinically relevant orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma through surgical tumor tissues injection with trocar.,J. Exp. Clin. Cancer Res. ,2010年,Vol.29 No.1,p.1-8
Attorney, Agent or Firm:
Kenichi Morita
Kenjiro Yamaguchi
Hironori Nagayama
Yuzaki Ozaki
Kenjiro Yamaguchi
Hironori Nagayama
Yuzaki Ozaki