Title:
A manufacturing method of a retinal ganglion cell
Document Type and Number:
Japanese Patent JP6039128
Kind Code:
B2
Abstract:
An object of the present invention is to provide a method of inducing pluripotent stem cells to differentiate into clinically applicable retinal ganglion cells. The present invention provides a method for inducing pluripotent stem cells to differentiate into retinal ganglion cells that can be used for clinical application. Such method is a method for producing retinal ganglion cells with elongated axons comprising the following steps: (a) a step of inducing pluripotent stem cells to differentiate into retinal progenitor cells via floating culture; (b) a step of inducing the retinal progenitor cells obtained in step (a) to differentiate into retinal ganglion cells via floating culture; and (c) a step of allowing axons to elongate via adhesion culture of the retinal ganglion cells obtained in step (b).
Inventors:
Noriyuki Higashi
Tanaka Taku
Tadashi Yokoi
Tanaka Taku
Tadashi Yokoi
Application Number:
JP2016500001A
Publication Date:
December 07, 2016
Filing Date:
August 07, 2015
Export Citation:
Assignee:
National Center for Child Health and Development
Senju Pharmaceutical Co., Ltd.
Senju Pharmaceutical Co., Ltd.
International Classes:
C12N5/0797; A61K35/30; A61P27/02; C12N5/0793; C12Q1/02; G01N33/15; G01N33/50
Domestic Patent References:
| JP2013509859A | 2013-03-21 | |||
| JP2013509859A | 2013-03-21 |
Foreign References:
| WO2013077425A1 | 2013-05-30 |
Other References:
JPN6016014358; ＲＩＡＺＩＦＡＲ Ｈａｍｉｄｒｅｚａ ｅｔ ａｌ．: 'Ｃｈｅｍｉｃａｌｌｙ ｉｎｄｕｃｅｄ ｓｐｅｃｉｆｉｃａｔｉｏｎ ｏｆｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ ｆｒｏｍ ｈｕｍａｎ ｅｍｂｒｙｏｎｉｃ ａｎｄ ｉｎｄｕｃｅｄｐｌｕｒｉｐｏｔｅｎ' Ｓｔｅｍ Ｃｅｌｌｓ Ｔｒａｎｓｌ Ｍｅｄ Ｖｏｌ．３，Ｎｏ．４, 201404, ４２４-４３２
JPN6016014359; ＴＡＮＡＫＡ Ｔａｋｕ ｅｔ ａｌ．: 'Ｇｅｎｅｒａｔｉｏｎ ｏｆ ｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ ｗｉｔｈｆｕｎｃｔｉｏｎａｌ ａｘｏｎｓ ｆｒｏｍ ｈｕｍａｎ ｉｎｄｕｃｅｄ ｐｌｕｒｉｐｏｔｅｎｔ ｓｔｅｍ ｃｅｌｌｓ．' ＳｃｉＲｅｐ． ５：８３４４, 20150210
JPN6016014360; ＢＯＳＣＯ Ａｌｅｊａｎｄｒａ ｅｔ ａｌ．: 'ＢＤＮＦ ａｎｄ ＮＴ-４ ｄｉｆｆｅｒｅｎｔｉａｌｌｙ ｍｏｄｕｌａｔｅｎｅｕｒｉｔｅ ｏｕｔｇｒｏｗｔｈ ｉｎ ｄｅｖｅｌｏｐｉｎｇ ｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ．' ＪＮｅｕｒｏｓｃｉ Ｒｅｓ Ｖｏｌ．５７， Ｎｏ．６, 19990915, ７５９-７６９
JPN6016014356; 瀧原祐史 ら: '培養網膜神経節細胞軸索流の動的画像解析' 日本眼科学会雑誌 Ｖｏｌ．１１５ 臨時増刊号, 20110415, ｐ．２５５ Ｏ２-２５１
JPN6016014357; 大鳥安正: '培養網膜神経節細胞の細胞死' 医学のあゆみ Ｖｏｌ．１８９， Ｎｏ．４, 199904, ｐ．２１９-２２２
JPN6016014358; ＲＩＡＺＩＦＡＲ Ｈａｍｉｄｒｅｚａ ｅｔ ａｌ．: 'Ｃｈｅｍｉｃａｌｌｙ ｉｎｄｕｃｅｄ ｓｐｅｃｉｆｉｃａｔｉｏｎ ｏｆｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ ｆｒｏｍ ｈｕｍａｎ ｅｍｂｒｙｏｎｉｃ ａｎｄ ｉｎｄｕｃｅｄｐｌｕｒｉｐｏｔｅｎ' Ｓｔｅｍ Ｃｅｌｌｓ Ｔｒａｎｓｌ Ｍｅｄ Ｖｏｌ．３，Ｎｏ．４, 201404, ４２４-４３２
JPN6016014359; ＴＡＮＡＫＡ Ｔａｋｕ ｅｔ ａｌ．: 'Ｇｅｎｅｒａｔｉｏｎ ｏｆ ｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ ｗｉｔｈｆｕｎｃｔｉｏｎａｌ ａｘｏｎｓ ｆｒｏｍ ｈｕｍａｎ ｉｎｄｕｃｅｄ ｐｌｕｒｉｐｏｔｅｎｔ ｓｔｅｍ ｃｅｌｌｓ．' ＳｃｉＲｅｐ． ５：８３４４, 20150210
JPN6016014360; ＢＯＳＣＯ Ａｌｅｊａｎｄｒａ ｅｔ ａｌ．: 'ＢＤＮＦ ａｎｄ ＮＴ-４ ｄｉｆｆｅｒｅｎｔｉａｌｌｙ ｍｏｄｕｌａｔｅｎｅｕｒｉｔｅ ｏｕｔｇｒｏｗｔｈ ｉｎ ｄｅｖｅｌｏｐｉｎｇ ｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ．' ＪＮｅｕｒｏｓｃｉ Ｒｅｓ Ｖｏｌ．５７， Ｎｏ．６, 19990915, ７５９-７６９
JPN6016014359; ＴＡＮＡＫＡ Ｔａｋｕ ｅｔ ａｌ．: 'Ｇｅｎｅｒａｔｉｏｎ ｏｆ ｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ ｗｉｔｈｆｕｎｃｔｉｏｎａｌ ａｘｏｎｓ ｆｒｏｍ ｈｕｍａｎ ｉｎｄｕｃｅｄ ｐｌｕｒｉｐｏｔｅｎｔ ｓｔｅｍ ｃｅｌｌｓ．' ＳｃｉＲｅｐ． ５：８３４４, 20150210
JPN6016014360; ＢＯＳＣＯ Ａｌｅｊａｎｄｒａ ｅｔ ａｌ．: 'ＢＤＮＦ ａｎｄ ＮＴ-４ ｄｉｆｆｅｒｅｎｔｉａｌｌｙ ｍｏｄｕｌａｔｅｎｅｕｒｉｔｅ ｏｕｔｇｒｏｗｔｈ ｉｎ ｄｅｖｅｌｏｐｉｎｇ ｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ．' ＪＮｅｕｒｏｓｃｉ Ｒｅｓ Ｖｏｌ．５７， Ｎｏ．６, 19990915, ７５９-７６９
JPN6016014356; 瀧原祐史 ら: '培養網膜神経節細胞軸索流の動的画像解析' 日本眼科学会雑誌 Ｖｏｌ．１１５ 臨時増刊号, 20110415, ｐ．２５５ Ｏ２-２５１
JPN6016014357; 大鳥安正: '培養網膜神経節細胞の細胞死' 医学のあゆみ Ｖｏｌ．１８９， Ｎｏ．４, 199904, ｐ．２１９-２２２
JPN6016014358; ＲＩＡＺＩＦＡＲ Ｈａｍｉｄｒｅｚａ ｅｔ ａｌ．: 'Ｃｈｅｍｉｃａｌｌｙ ｉｎｄｕｃｅｄ ｓｐｅｃｉｆｉｃａｔｉｏｎ ｏｆｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ ｆｒｏｍ ｈｕｍａｎ ｅｍｂｒｙｏｎｉｃ ａｎｄ ｉｎｄｕｃｅｄｐｌｕｒｉｐｏｔｅｎ' Ｓｔｅｍ Ｃｅｌｌｓ Ｔｒａｎｓｌ Ｍｅｄ Ｖｏｌ．３，Ｎｏ．４, 201404, ４２４-４３２
JPN6016014359; ＴＡＮＡＫＡ Ｔａｋｕ ｅｔ ａｌ．: 'Ｇｅｎｅｒａｔｉｏｎ ｏｆ ｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ ｗｉｔｈｆｕｎｃｔｉｏｎａｌ ａｘｏｎｓ ｆｒｏｍ ｈｕｍａｎ ｉｎｄｕｃｅｄ ｐｌｕｒｉｐｏｔｅｎｔ ｓｔｅｍ ｃｅｌｌｓ．' ＳｃｉＲｅｐ． ５：８３４４, 20150210
JPN6016014360; ＢＯＳＣＯ Ａｌｅｊａｎｄｒａ ｅｔ ａｌ．: 'ＢＤＮＦ ａｎｄ ＮＴ-４ ｄｉｆｆｅｒｅｎｔｉａｌｌｙ ｍｏｄｕｌａｔｅｎｅｕｒｉｔｅ ｏｕｔｇｒｏｗｔｈ ｉｎ ｄｅｖｅｌｏｐｉｎｇ ｒｅｔｉｎａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ．' ＪＮｅｕｒｏｓｃｉ Ｒｅｓ Ｖｏｌ．５７， Ｎｏ．６, 19990915, ７５９-７６９
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Nobuko Fukami
Satoshi Fujita
Nobuko Fukami