Title:
The measuring method and the kit for measurement of Spingo myelin
Document Type and Number:
Japanese Patent JP6071883
Kind Code:
B2
Abstract:
Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefor. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.
Inventors:
Kimura Gohide
Miyauchi alone
Hideyuki Kuwata
Miyauchi alone
Hideyuki Kuwata
Application Number:
JP2013526840A
Publication Date:
February 01, 2017
Filing Date:
July 25, 2012
Export Citation:
Assignee:
Kyowa Medex Co., Ltd.
International Classes:
C12Q1/44; C12Q1/26; C12Q1/28; C12Q1/30; C12Q1/32
Domestic Patent References:
| JP2009519713A | ||||
| JP9023897A | ||||
| JP6197794A | ||||
| JP6197793A |
Foreign References:
| WO2009048143A1 | ||||
| WO2001080903A1 |
Other References:
J. Biochem.,1979年,Vol. 85,pp. 79-95
協和メデックス株式会社,リン脂質キット 自動分析装置用 デタミナーL PL,カタログ添付文書,2008年 4月
協和メデックス株式会社,リン脂質キット 自動分析装置用 デタミナーL PL,カタログ添付文書,2008年 4月
Attorney, Agent or Firm:
Masaki Hirota