Title:
目的とする配列を連結するための方法
Document Type and Number:
Japanese Patent JP4651619
Kind Code:
B2
Abstract:
Multiplex overlap-extension RT-PCR provides an efficient method of linking two or more nucleotide sequences encoding for domains or subunits of a heteromeric protein, in a single reaction. Especially, the linkage of variable region encoding sequences from e.g. immunoglobulins, T cell receptors or B cell receptors is eased with the method of the present invention. This allows for a more efficient way of generating libraries of variable region encoding sequences. The capability to perform the multiplex overlap-extension RT-PCR using template derived from an isolated single cell enables the generation of cognate pair libraries in a high-throughput format.
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Inventors:
Martin B. Orexwicz
Lars S. Nielsen
Peter S. Anacen
Margit Ho Hansen
Lars S. Nielsen
Peter S. Anacen
Margit Ho Hansen
Application Number:
JP2006526523A
Publication Date:
March 16, 2011
Filing Date:
September 17, 2004
Export Citation:
Assignee:
SYMPHOGEN A/S
International Classes:
C12N15/09; C07K16/00; C07K16/12; C12N5/10; C12N15/10; C12N15/13; C12P21/02; C12P21/08; C40B40/08
Domestic Patent References:
| JP6509473A |
Foreign References:
| WO2001089563A1 |
Other References:
Nucl. Acids Res., vol. 28, e85 (total 7 pages) (2000)
Nucl. Acids Res., vol. 20, pages 3831-3837 (1992)
J. Mol. Biol., vol. 358, pages 764-772 (2006)
BioTechniques, vol. 23, pages 518-524 (1997)
Nucl. Acids Res., vol. 20, pages 3831-3837 (1992)
J. Mol. Biol., vol. 358, pages 764-772 (2006)
BioTechniques, vol. 23, pages 518-524 (1997)
Attorney, Agent or Firm:
Mitsuo Tanaka
Tadayuki Motoyama
Kenji Tomita
Tadayuki Motoyama
Kenji Tomita