Title:
熱安定性ＣＡＳ９ヌクレアーゼ
Document Type and Number:
Japanese Patent JP7283698
Kind Code:
B2
Abstract:
Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.
Inventors:
Van der Oust, John
van kranenburg, richard
Bosma, Elecke Fenna
Mougiacos, Ioannis
Mohan Raju, Praltana
van kranenburg, richard
Bosma, Elecke Fenna
Mougiacos, Ioannis
Mohan Raju, Praltana
Application Number:
JP2019533098A
Publication Date:
May 30, 2023
Filing Date:
December 14, 2017
Export Citation:
Assignee:
Wageningen Universitet
Stitching Fall de Technish Wetenschapen
Stitching Fall de Technish Wetenschapen
International Classes:
C12N15/09; C12N15/11; C12N15/63
Domestic Patent References:
| JP2018522544A | ||||
| JP2020504603A | ||||
| JP2020505000A | ||||
| JP2020510410A |
Foreign References:
| WO2016186946A1 |
Other References:
'ACCESSION: A0A178TEJ9, entry version 3, RecName: CRISPR-associated endonuclease Cas9',UniProt [online],2016年11月02日,,[retrieved 27-DEC-2021] which retrieved from the internet:
J Control Release, 2016 Aug 4, vol. 244, pp. 139-148
Trends Biotechnol, 2016 Jul, vol. 34, no. 7, pp. 575-587
Molecular Cell,2016年04月07日,vol.62,p.137-147
Genome Biology,2015年,16:253,1-13
Nat Commun, 2017 Nov 21, vol. 8, no. 1, article no. 1647 (pp. 1-11)
J Control Release, 2016 Aug 4, vol. 244, pp. 139-148
Trends Biotechnol, 2016 Jul, vol. 34, no. 7, pp. 575-587
Molecular Cell,2016年04月07日,vol.62,p.137-147
Genome Biology,2015年,16:253,1-13
Nat Commun, 2017 Nov 21, vol. 8, no. 1, article no. 1647 (pp. 1-11)
Attorney, Agent or Firm:
Hiroshi Kobayashi
Norio Omori
Hisashi Fujita
Norio Omori
Hisashi Fujita