Title:
ALTERING ENZYME BALANCE THROUGH FERMENTATION CONDITIONS
Document Type and Number:
WIPO Patent Application WO/2010/127219
Kind Code:
A3
Abstract:
This present disclosure relates to methods for improved production of proteins from a cell culture, particularly to culture components and conditions that can preferentially increase the expression of proteins produced from genes under the control of xylanase gene promoter sequences. The improved methods can be used for the production of enzyme compositions with enhanced xylanase and hemicellulolytic activity.
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Inventors:
KELLEY AARON (US)
LIN CHUANBIN (US)
MITCHINSON COLIN (US)
LIN CHUANBIN (US)
MITCHINSON COLIN (US)
Application Number:
PCT/US2010/033125
Publication Date:
March 03, 2011
Filing Date:
April 30, 2010
Export Citation:
Assignee:
DANISCO US INC (US)
KELLEY AARON (US)
LIN CHUANBIN (US)
MITCHINSON COLIN (US)
KELLEY AARON (US)
LIN CHUANBIN (US)
MITCHINSON COLIN (US)
International Classes:
C12N9/42; C12N1/14; C12N9/58; C12P19/12; C12P19/14
Domestic Patent References:
| WO2008008070A2 | 2008-01-17 | |||
| WO2004035070A1 | 2004-04-29 |
Other References:
P. K. FOREMAN ET AL: "Transcriptional Regulation of Biomass-degrading Enzymes in the Filamentous Fungus Trichoderma reesei", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 34, 22 August 2003 (2003-08-22), pages 31988 - 31997, XP002614970
ZEILINGER SUSANNE ET AL: "Different inducibility of expression of the two xylanase genes xyn1 and xyn2 in Trichoderma reesei", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC, US, vol. 271, no. 41, 1 January 1996 (1996-01-01), pages 25624 - 25629, XP002196924, ISSN: 0021-9258, DOI: 10.1074/JBC.271.41.25624
ILMEN M ET AL: "Regulation of cellulase gene expression n the filamentous fungs trichoderma reesei", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 63, no. 4, 1 April 1997 (1997-04-01), pages 1298 - 1306, XP009140403, ISSN: 0099-2240
MARGOLLES-CLARK E ET AL: "Expression patterns of ten hemicellulase genes of the filamentous fungus Trichoderma reesei on various carbon sources", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 57, no. 1-3, 16 September 1997 (1997-09-16), pages 167 - 179, XP004097715, ISSN: 0168-1656, DOI: 10.1016/S0168-1656(97)00097-7
ZEILINGER SUSANNE ET AL: "Different inducibility of expression of the two xylanase genes xyn1 and xyn2 in Trichoderma reesei", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC, US, vol. 271, no. 41, 1 January 1996 (1996-01-01), pages 25624 - 25629, XP002196924, ISSN: 0021-9258, DOI: 10.1074/JBC.271.41.25624
ILMEN M ET AL: "Regulation of cellulase gene expression n the filamentous fungs trichoderma reesei", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 63, no. 4, 1 April 1997 (1997-04-01), pages 1298 - 1306, XP009140403, ISSN: 0099-2240
MARGOLLES-CLARK E ET AL: "Expression patterns of ten hemicellulase genes of the filamentous fungus Trichoderma reesei on various carbon sources", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 57, no. 1-3, 16 September 1997 (1997-09-16), pages 167 - 179, XP004097715, ISSN: 0168-1656, DOI: 10.1016/S0168-1656(97)00097-7
Attorney, Agent or Firm:
KOLMAN, Michael (925 Page Mill RoadPalo Alto, California, US)
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