Title:
ANALYSIS METHOD AND ANALYZER
Document Type and Number:
WIPO Patent Application WO/2019/198812
Kind Code:
A1
Abstract:
Provided is an analysis method comprising: a step in which four or more enzyme substrates which are reduced, deficient, or not working properly in the body of a patient suffering from lysosome disease are reacted with enzymes contained in samples obtained from living organisms among the four or more enzymes to produce reaction products; separating the reaction products by liquid chromatography; and detecting the separated reaction products by mass spectrometry, wherein the four or more enzymes include iduronic acid-2-sulfatase.
Inventors:
TANAKA MISA (JP)
WATANABE JUN (JP)
KOBAYASHI HIRONORI (JP)
IIDA TETSUO (JP)
WATANABE JUN (JP)
KOBAYASHI HIRONORI (JP)
IIDA TETSUO (JP)
Application Number:
PCT/JP2019/015951
Publication Date:
October 17, 2019
Filing Date:
April 12, 2019
Export Citation:
Assignee:
SHIMADZU CORP (JP)
NATIONAL UNIV CORPORATION SHIMANE UNIV (JP)
NATIONAL UNIV CORPORATION SHIMANE UNIV (JP)
International Classes:
G01N30/72; C12M1/34; C12Q1/34; C12Q1/44; G01N27/62; G01N30/86; G01N30/88
Foreign References:
| JP2014532442A | 2014-12-08 | |||
| JP2016529910A | 2016-09-29 |
Other References:
LIU, YANG ET AL.: "Multiplex Tandem Mass Spectrometry Enzymatic Activity Assay for Newborn Screening of the Mucopolysaccharidoses and Type 2 Neuronal Ceroid Lipofuscinosis", CLINICAL CHEMISTRY, vol. 63, no. 6, 2017, pages 1118 - 1126, XP055521743, DOI: 10.1373/clinchem.2016.269167
SPACIL, ZDENEK ET AL.: "High-Throughput Assay of 9 Lysosomal Enzymes for Newborn Screening", CLINICAL CHEMISTRY, vol. 59, no. 3, 11 January 2013 (2013-01-11) - 1 March 2013 (2013-03-01), pages 502 - 511, XP055192193
GUCCIARDI, ANTONINA ET AL.: "A column-switching HPLC-MS/MS method for mucopolysaccharidosis type I analysis in a multiplex assay for the simultaneous newborn screening of six lysosomal storage disorders", BIOMEDICAL CHROMATOGRAPHY, vol. 28, 2014, pages 1131 - 1139, XP055304261, DOI: 10.1002/bmc.3133
OHIRA, MARI ET AL.: "Quantification of 11 enzyme activities of lysosomal storage disorders using liquid chromatography-tandem mass spectrometry", MOLECULAR GENETICS AND METABOLISM REPORTS, vol. 17, 7 September 2018 (2018-09-07), pages 9 - 15, XP055521737, DOI: 10.1016/j.ymgmr.2018.08.005
SPACIL, ZDENEK ET AL.: "High-Throughput Assay of 9 Lysosomal Enzymes for Newborn Screening", CLINICAL CHEMISTRY, vol. 59, no. 3, 11 January 2013 (2013-01-11) - 1 March 2013 (2013-03-01), pages 502 - 511, XP055192193
GUCCIARDI, ANTONINA ET AL.: "A column-switching HPLC-MS/MS method for mucopolysaccharidosis type I analysis in a multiplex assay for the simultaneous newborn screening of six lysosomal storage disorders", BIOMEDICAL CHROMATOGRAPHY, vol. 28, 2014, pages 1131 - 1139, XP055304261, DOI: 10.1002/bmc.3133
OHIRA, MARI ET AL.: "Quantification of 11 enzyme activities of lysosomal storage disorders using liquid chromatography-tandem mass spectrometry", MOLECULAR GENETICS AND METABOLISM REPORTS, vol. 17, 7 September 2018 (2018-09-07), pages 9 - 15, XP055521737, DOI: 10.1016/j.ymgmr.2018.08.005
Attorney, Agent or Firm:
NAGAI, Fuyuki et al. (JP)
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