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Title:
BACILLUS THURINGIENSIS HYBRID TOXINS
Document Type and Number:
WIPO Patent Application WO2001034811
Kind Code:
A3
Abstract:
Bacillus thuringiensis hybrid toxin fragment comprising structural domains I, II and III in this order starting from the N-terminal, wherein the domains are derived from at least two different Cry protein, domain I is domain I of any Bacillus thuringiensis Cry protein or a part of said domain or a peptide substantially similar to said domain, domain II is domain II or Cry 1 Ia or a part of said domain or a peptide substantially similar to said domain, and domain III is domain III of Cry1Ba or a part of said domain or a peptide substantially similar domain.

Inventors:
DE MAAGD RUDOLF AART (NL)
BOSCH HENDRIK JAN (NL)
Application Number:
PCT/NL2000/000817
Publication Date:
December 27, 2001
Filing Date:
November 09, 2000
Export Citation:
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Assignee:
PLANT RES INT BV (NL)
MAAGD RUDOLF AART DE (NL)
BOSCH HENDRIK JAN (NL)
International Classes:
A01H5/00; A01N63/00; C07K14/325; C12N15/32; C12N15/62; (IPC1-7): C12N15/32; C07K14/325; C12N15/62; A01H5/00; A01N63/00
Domestic Patent References:
WO1999050293A11999-10-07
WO1995006730A11995-03-09
Other References:
VISSER ET AL: "Domain-function studies of Bacillus thuringiensis crystal proteins: a genetic approach", ENTWISTLE, P.F. (EDS) : "BACILLUS THURINGIENSIS, AN ENVIRONMENTAL BIOPESTICIDE: THEORY AND PRACTICE", 1993, GB,CHICHESTER, WILEY & SONS, pages 71 - 88, XP002086356
ELY S: "THE ENGINEERING OF PLANTS TO EXPRESS BACILLUS THURINGIENSIS DELTA-ENDOTOXINS", ENTWISTLE, P.F. (EDS) : "BACILLUS THURINGIENSIS, AN ENVIRONMENTAL BIOPESTICIDE: THEORY AND PRACTICE", 1993, GB,CHICHESTER, WILEY & SONS, pages 105 - 124, XP002054693
BRADLEY D ET AL: "The Insecticidal CryIB Crystal Protein of Bacillus thuringiensis ssp. thuringiensis Has Dual Specificity to Coleopteran and Lepidopteran Larvae.", JOURNAL OF INVERTEBRATE PATHOLOGY, vol. 65, no. 2, 1995, pages 162 - 173, XP000891272, ISSN: 0022-2011
MAAGD DE R A: "DIFFERENT DOMAINS OF BACILLUS THURINGIENSIS DELTA-ENDOTOXINS CAN BIND TO INSECT MIDGUT MEMBRANE PROTEINS ON LIGAND BLOTS", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 62, no. 8, August 1996 (1996-08-01), pages 2753 - 2757, XP002061801, ISSN: 0099-2240
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