Title:
BONE MATRIX, THE UTILIZATION THEREOF, THERAPEUTIC PREPARATION AND KIT
Document Type and Number:
WIPO Patent Application WO1999027977
Kind Code:
A3
Abstract:
The invention relates to a bone matrix which can be obtained by: (a) starting out from an explantation and obtaining osteogenic precursor cells or starting out from an osteogenic cell line; (b) optionally expanding the obtained osteogenic precursor cells or the osteogenic cell line in-vitro; (c) permitting the osteogenic precursor cells to propagate, said cells being obtained according to (a) or expanded according to (b), or permitting the cell line to propagate, said cell line being inserted according to (a) or expanded according to (b), and optionally obtaining osteogenic cells; (d) separate from step (c), permitting the osteogenic cells to synthesize in-vitro in an extracellular matrix, said cells being obtained according to (a) or expanded according to (b), or permitting the cell line to synthesize in-vitro in an extracellular matrix, said cell line being introduced according to (a) or expanded according to (b); (e) optionally liberating the synthesized matrix from cells and optionally obtaining a cell-free bone matrix; (f) populating the obtained cell-free bone matrix once more with osteogenic cells which have been obtained according to (c) and optionally obtaining a populated bone matrix by using osteogenic cells.
Inventors:
MAYER HUBERT (DE)
GROSS GERHARD (DE)
ROHDE MANFRED (DE)
JAEGER VOLKER (DE)
SCHLIEPHAKE HENNING (DE)
BARTHOLD MARC (DE)
GROSS GERHARD (DE)
ROHDE MANFRED (DE)
JAEGER VOLKER (DE)
SCHLIEPHAKE HENNING (DE)
BARTHOLD MARC (DE)
Application Number:
PCT/EP1998/007708
Publication Date:
September 10, 1999
Filing Date:
November 30, 1998
Export Citation:
Assignee:
BIOTECHNOLOG FORSCHUNG GMBH (DE)
MAYER HUBERT (DE)
GROSS GERHARD (DE)
ROHDE MANFRED (DE)
JAEGER VOLKER (DE)
SCHLIEPHAKE HENNING (DE)
BARTHOLD MARC (DE)
MAYER HUBERT (DE)
GROSS GERHARD (DE)
ROHDE MANFRED (DE)
JAEGER VOLKER (DE)
SCHLIEPHAKE HENNING (DE)
BARTHOLD MARC (DE)
International Classes:
A61L27/00; A61L27/38; C12N5/06; C12N5/077; A61K35/12; (IPC1-7): A61L27/00; C12N5/06
Domestic Patent References:
| WO1995032709A1 | 1995-12-07 | |||
| WO1998017791A1 | 1998-04-30 | |||
| WO1998000183A2 | 1998-01-08 | |||
| WO1995033821A1 | 1995-12-14 |
Foreign References:
| EP0358506A2 | 1990-03-14 |
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