Title:
BORON ABSORPTION-PROMOTING GENE
Document Type and Number:
WIPO Patent Application WO/2006/106678
Kind Code:
A1
Abstract:
It is intended to provide boron absorption-promoting gene relating to the absorption of boron by which the incorporation of born from the environment and boron transport in vivo can be more efficiently controlled. A gene NIP5;1, which shows an increase in the accumulation of mRNA in Arabidopsis root under low-boron conditions, is found out. Further, a NIP5;1 prmoter::GUS Arabidopsis transformant is constructed and grown in media with different boron concentrations. Then, the GUS activities are measured. As a result, a stronger GUS activity is observed in boron absorption promotion in a medium having the lower boron concentration, which indicates that the NIP5;1 promoter activity is induced under low-boron conditions. As the results of studies on the tissue-specificity of the NIP5;1 expression with the use of the NIP5;1 prmoter::GUS Arabidopsis transformant, it is found out that this gene is strongly expressed in epidermal cells in root elongation areas.
Inventors:
FUJIWARA TORU (JP)
TAKANO JUNPEI (JP)
WADA MOTOKO (JP)
TAKANO JUNPEI (JP)
WADA MOTOKO (JP)
Application Number:
PCT/JP2006/306311
Publication Date:
October 12, 2006
Filing Date:
March 28, 2006
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
FUJIWARA TORU (JP)
TAKANO JUNPEI (JP)
WADA MOTOKO (JP)
FUJIWARA TORU (JP)
TAKANO JUNPEI (JP)
WADA MOTOKO (JP)
International Classes:
C12N15/00; A01H5/00; C07K14/415; C12N1/19; C12N5/10; C12N15/09; C12Q1/02; G01N33/15; G01N33/50
Other References:
QUIGLEY F. ET AL.: "From genome to function: the Arabidopsis aquaporins", GENOME BIOL., vol. 3, no. 1, 7 December 2001 (2001-12-07), pages RESEARCH0001.1 - RESEARCH0001.17, XP021021078
DATABASE GENBANK [online] 6 November 1997 (1997-11-06), NEWMAN T. ET AL.: "Definition: 3 Lambda-PRL1 Arabidopsis thaliana cDNA clone S15ATM, mRNA sequence", XP003016075, accession no. EMBL Database accession no. (T04053)
FUJIWARA T. ET AL.: "Shokubutsu ni yoru Hoso no Kyushu to Yuso", (JAPAN SCIENCE AND TECHNOLOGY CORP. SAKIGAKE 21 KENKYU), KIKAN HIRYO, no. 96, 2003, pages 71 - 76, XP003016076
DORDAS C. ET AL.: "Permeability and channel-mediated transport of boric acid across membrane vesicles isolated from squash roots", PLANT PHYSIOL., vol. 124, no. 3, 2000, pages 1349 - 1361, XP003016077
EL KARKOURI K. ET AL.: "MIPDB: a relational database dedicated to MIP family proteins", BIOL. CELL, vol. 97, no. 7, July 2005 (2005-07-01), pages 535 - 543, XP003016078
DATABASE GENBANK [online] 6 November 1997 (1997-11-06), NEWMAN T. ET AL.: "Definition: 3 Lambda-PRL1 Arabidopsis thaliana cDNA clone S15ATM, mRNA sequence", XP003016075, accession no. EMBL Database accession no. (T04053)
FUJIWARA T. ET AL.: "Shokubutsu ni yoru Hoso no Kyushu to Yuso", (JAPAN SCIENCE AND TECHNOLOGY CORP. SAKIGAKE 21 KENKYU), KIKAN HIRYO, no. 96, 2003, pages 71 - 76, XP003016076
DORDAS C. ET AL.: "Permeability and channel-mediated transport of boric acid across membrane vesicles isolated from squash roots", PLANT PHYSIOL., vol. 124, no. 3, 2000, pages 1349 - 1361, XP003016077
EL KARKOURI K. ET AL.: "MIPDB: a relational database dedicated to MIP family proteins", BIOL. CELL, vol. 97, no. 7, July 2005 (2005-07-01), pages 535 - 543, XP003016078
Attorney, Agent or Firm:
Hirota, Masanori (8-5 Akasaka 2-chom, Minato-ku Tokyo, JP)
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