BUFFER COMPOSITION FOR NUCLEIC ACID ISOLATION FOR COLUMN-BASED ONE-TUBE PCR, AND USE THEREOF

Title:

BUFFER COMPOSITION FOR NUCLEIC ACID ISOLATION FOR COLUMN-BASED ONE-TUBE PCR, AND USE THEREOF

Document Type and Number:

WIPO Patent Application WO/2022/154214

Kind Code:

A1

Abstract:

The present invention relates to a nucleic acid isolation buffer composition, a nucleic acid isolation kit in which a membrane filter comprising same is provided, and a nucleic acid isolation method, wherein the nucleic acid isolation buffer composition has excellent compatibility with membrane filters of various materials, and enables the excellent isolation of nucleic acids from various samples, and, particularly, a nucleic acid isolation kit comprising same enables column-based one-tube PCR, and a nucleic acid isolation method using same enables the direct application of the membrane filter, on which nucleic acids are immobilized, to PCR without a separate elution process. Therefore, the nucleic acid isolation composition, the kit and the isolation method according to the present invention can be effectively used in rapid diagnosis onsite.

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Inventors:

RYU SEONG HO (KR)

Application Number:

PCT/KR2021/014744

Publication Date:

July 21, 2022

Filing Date:

October 20, 2021

Export Citation:

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Assignee:

GENE2US CO (KR)
UNIV SOONCHUNHYANG IND ACAD COOP FOUND (KR)

International Classes:

C12N15/10; C12Q1/6806; C12Q1/686

Foreign References:

KR100486179B1	2005-05-03
KR100845971B1	2008-07-11
KR101885039B1	2018-08-03
KR20150057120A	2015-05-28
KR102041333B1	2019-11-06
KR102291738B1	2021-08-20

Other References: