Title:
CARDIOVASCULAR CELL CO-CULTURE MEDIUM AND METHOD OF GROWING MULTIPLE CARDIOVASCULAR CELL TYPES
Document Type and Number:
WIPO Patent Application WO/2023/133441
Kind Code:
A3
Abstract:
Described herein is a unique cell culture media that allows optimal growth of multiple cell types in the same system such as an organ-on-chip or organoid. Also described herein is use of the cell culture media in growing multiple cardiovascular cell types simultaneously in a single device or producing induced pluripotent stem cell (iPSC)-derived cardiac organoids containing iPSC derived-endothelial cells (iPSC-ECs) and iPSC derived- cardiomyocytes (iPSC-CMs). In some embodiments, a formulation for the cell culture media includes a base medium including glucose, a pH indicator, salts, amino acids, and vitamins; recombinant human albumin; L-ascorbic acid 2-phosphate; and at least one or more of insulin, vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), a synthetic analogue of insulin-like growth factor (IGF)-I, heparin, or hydrocortisone.
Inventors:
SHARMA ARUN (US)
SVENDSEN CLIVE N (US)
SVENDSEN CLIVE N (US)
Application Number:
PCT/US2023/060137
Publication Date:
August 17, 2023
Filing Date:
January 05, 2023
Export Citation:
Assignee:
CEDARS SINAI MEDICAL CENTER (US)
International Classes:
C12N5/077; A61K35/34; A61K9/08; A61P9/00
Foreign References:
| US20190117701A1 | 2019-04-25 | |||
| US20160340652A1 | 2016-11-24 |
Other References:
ANONYMOUS: "RPMI 1640, Formulation", CORNING, 1 January 2018 (2018-01-01), XP093085462, Retrieved from the Internet [retrieved on 20230925]
ERMAK THOMAS H, FLIES AMANDA: "Quantitation of LONG ® R 3 IGF-I during production and purification of recombinant proteins from cell culture", 18 February 2019 (2019-02-18), XP093085461, Retrieved from the Internet [retrieved on 20230925]
ERMAK THOMAS H, FLIES AMANDA: "Quantitation of LONG ® R 3 IGF-I during production and purification of recombinant proteins from cell culture", 18 February 2019 (2019-02-18), XP093085461, Retrieved from the Internet
Attorney, Agent or Firm:
LEE, Harry Sung et al. (US)
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